- Detection of HBV DNA in Needle Biopsied Paraffin Embedded Liver Tissues of Chronic Hepatitis B Patients by PCR: Comparison with Serological and Immunohistochemical Studies.
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Hye Soo Lee, Kahng Yeul Oh, Joo Heon Kim, Yoon Jeong Kim, Sam Im Choi, Dong Geun Lee, Sang Ho Kim
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Korean J Pathol. 1996;30(6):495-504.
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 Abstract
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- In this study, the prevalence of Hepatitis B virus(HBV) DNA in the needle biopsied paraffin embedded liver tissues of chronic hepatitis B patients by rapid nested PCR was examined. DNA was extracted by NaOH with boiling, and amplified by rapid air thermocycler with glass capillary tubes and nested PCR with two primer sets specific for the surface and the core genes of HBV. The PCR results were compared to that of serum HBeAg, serum HBV DNA by dot blot hybridization with a radioactive DNA probe, and tissue immunohistochemical (HBsAg/ HBcAg) studies. Among 44 patients with chronic hepatitis with serum HBsAg positivity, HBV DNA could be detected by PCR in 43 liver tissues (98%).
This results were comparable to the positive rates of 94%(31/33) for serum HBV DNA, 80%(35/44) for serum HBeAg, and 59%(26/44) and 75%(33/44) for tissue HBsAg and HBcAg, respectively. The accordance rate between tissue PCR and serum DNA probe testing was 91%. The results indicate that HBV DNA detection by rapid nested PCR of paraffin embedded liver tissues by needle biopsy is a more sensitive method to detect the HBV DNA carrier than the serum HBeAg or tissue HBsAg/HBcAg status, and is well correlated with the result of serum HBV DNA probe testing. Therefore this method is a practical indicator for the diagnosis and replication status in retrospective analysis.
- An Experimental Study of DMBA (9,10-Dimethyl-1,2-Benzanthracene) Induced Knee Joint Tumors in the Rats.
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Myung Jae Kang, Dong Geun Lee, Sam Im Choi, Sang Ho Kim
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Korean J Pathol. 1988;22(4):424-434.
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- For the morphological analysis of DMBA (9,10-diemethyl-1,2-benzanthracene) induced tumor, thirty Sprague-Dawley rats were received 0.1 ml of a 2% paraffin solution of DMBA into the knee joint cavity, which was repeated three times at an interval of 4 weeks. The induced tumor masses were removed at the 12th week after the first injection. Histological and histochemical examinations (H & E, PAS, alcian blue, Van Gieson, prussian blue, reticulin, PTAH stain) and enzyme histochemical examinations (acid phosphatase, alkaline phosphatase, alpha-naphthyl acetate esterase) were performed. The results were as follows: 1) By the 12th week after the first injection of DMBA, the tumor incidence rate was 20 percent. 2) On histological and histochemical examination, most of the induced tumor disclosed the features of the fibrous histiocytoma originating from mesenchymal cells, and the remains sweat gland adenoma and adenocarcinoma originating from epithelial cells. 3) On enzyme histochemical examination, most of the mesenchymal cell-derived tumor cells showed positive reactions for acid phosphatase and alpha-naphthyl acetate esterase, which were similar characteristic features of enzyme stains as shown in the component cells of fibrous histiocytoma.
- Immunohistochemical Study on the Tissue Localization of Human Chorionic Gonadotropin (HCG), Human Placental Lactogen (HPL), and Pregnancy Specific beta-1 Glycoprotein (SP-1).
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Soon Lee, Sang Soo Park, Sam Im Choi, Sang Ho Kim
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Korean J Cytopathol. 1985;19(4):402-412.
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Abstract
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- An immunohistochemical study on the tissue localization of human chorionic gonadotropin (HCG), human placental lactogen (HPL), and human pregnancy specific bata-1 glycoprotein (SP-1) in the non-neoplastic (5 incomplete abortion, 5 ectopic pregnancy) and neoplastic (hydatidiform mole 3 cases, 5 ectopic pregnancy) and neoplastic (hydatidiform mole 3 cases, choriocarcinoma 2 cases) gestational status, and germ cell tumors of the ovary (dysgerminoma 2 cases, immature teratoma 1 case) and the testis (seminoma 2 cases, endodermal sinus tumor 1 case) by means of sensitive peroxidase-antiperoxidase method. The results were as follows. 1) Cytoplasmic HCG, HPL, and SP-1 were readily identified in syncytiotrophoblasts of chorionic villi of the incomplete abortion and ectopic pregnancy, as moderate to strong positive reactions. In the cytoplasm of the neoplastic syncytiotrophblasts of hydatidiform mole and choriocarcinoma, positive reactions were stronger than in non-neoplastic syncytiotrophblasts. but the cytotrophoblasts and villous stromal cells showed negative reactions to these hormones. 2) All of the tumor cells of dysgerminoma and immature teratoma of the ovary, and seminoma and endodermal sinus tumor of the testis showed negative reactions to HCG, HPL, and SP-1. According to these results, HCG, HPL, and SP-1 are specifically synthesized and secreted by normal or neoplastic syncytiotrophoblasts Malignant tumors originated from other cells or tissues than trophoblastic cells show elevated serum concentration of these hormones are only evident in the presence of syncytiotrophoblastic giant cells among the tumor cells.
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